蔡志强,丁国富,李云飞,范勇洪,王勤章

• 1:石河子大学医学院
• 2:石河子大学医学院第一附属医院泌尿外科

摘要(Abstract)：

目的:观察体外高糖环境下Cajal样细胞(interstitial cells of Cajal-like,ICCs)c-kit mRNA表达变化。方法:胶原酶消化法原代分离培养豚鼠膀胱ICCs,加入含葡萄糖浓度分别为5mmol/L、15mmol/L、30mmol/L培养基培养24h、48h、72h后,应用逆转录-聚合酶链式反应(RT-PCR)法检测高糖环境下c-kit基因mRNA表达水平的变化。结果:RT-PCR电泳经分析显示15mmol/L、30mmol/L组ICCs c-kit mRNA表达较5mmol/L组降低(P<0.01),72h组较24h组、48h组表达降低。结论:高糖环境造成ICCs c-kit基因mRNA表达水平降低,可导致ICCs的SCF/c-kit信号通路异常,ICCs功能及结构障碍,可能是糖尿病膀胱病变的发病机制之一。

关键词(KeyWords)： Cajal样细胞;c-kit基因;高糖;糖尿病

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No.30860281)

作者(Author): 蔡志强,丁国富,李云飞,范勇洪,王勤章

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